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Purple-Jelley Hyaluronic Acid assay kit

Catalog No : H1000, H2000

The Purple-Jelley assay kit is an easy-to-use, dye-based, colorimetric method for measurement of hyaluronic acid (HA) / hyaluronan. The kit has been optimised for quantitative analysis of HA from tissue-derived samples.
Colorimetric Detection (655nm) (Endpoint)
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Specifications

Assay range : 10 - 100µg/ml

Limit of Detection : 10µg/ml

Detection Method : Colorimetric Detection (655nm) (Endpoint)

Measurements per kit : 100 in total (allows a maximum of 46 samples to be run in duplicate alongside a standard curve).

Suitable Samples : 

In-vivo: Hyaluronic acid purified from in-vivo tissues. The kit protocol involves extraction and purification of hyaluronic acid prior to reaction with the Purple-Dye reagent.

In-vitro:Measurement of hyaluronic acid from in-vitro cellular material is possible, but may require adaption of the protocol. Please contact us for further information.

Precautions : This kit is designed for research use only. Not for use in diagnostic procedures.
Kit requires access to a centrifuge, as well as a spectrophotometer/colorimeter capable of colorimetric, absorbance detection at 655nm.
Specific sample preparation protocols may require customer to provide further reagents, consult assay manual for further information.

 

Purple-Jelley Hyaluronic acid kit contents:‍

1. Purple-Jelley Dye Reagent (1x 20ml)

2. Hyaluronan Reference Standard (1x 5ml, 0.2mg/ml soluble Hyaluronic Acid)

3. Precipitating Reagent (2x 34ml)

4. Sodium Chloride (1x 20ml)

5. Cetylpyridinium Chloride (1x 20ml)

6. TRIS-buffered Saline (5x tablets)

7. 2ml screw-cap tubes for preparation of samples.

8. Assay kit manual

NB: Additional reagents may be required for sample preparation prior to assay. Consult manual or contact us for further details.

 

Product Codes

H1000 Purple-Jelley Hyaluronan Assay, (100 assays) [Standard Kit]

‍H2000 Purple-Jelley Hyaluronan Assay, (400 assays) [MultiPack]