HOME
R&D
ALL

Biocolor

Sircol™ - Insoluble Collagen assay kit

Catalog No : S2000, S6000

As collagens age, they become increasingly crosslinked and insoluble, characteristics necessary for the structural role they play in tissues such as bone. Biocolor's Sircol™ INSOLUBLE Collagen Kit is an easy-to-use dye-binding assay that can be used to accurately measure the amount of insoluble collagen content in both in-vivo and in-vitro tissues.
Colorimetric Detection (556nm) (Endpoint)
제품문의

Specifications 

Assay range : 100 - 1000 µg/ml

Limit of Detection : 100µg/ml

Detection Method : Colorimetric Detection (556nm) (Endpoint)

Measurements per kit : 110 in total (allows a maximum of 46 samples to be run in duplicate alongside a standard curve).

Suitable Samples : 

The assay can be used to assess the rate of production of newly laid down collagen fibres during periods of rapid growth, development, tissue repair, remodeling and wound healing. Sources of material includes tissues, bone and calcified tissue.

*Insoluble collagens must be converted into soluble form prior to assay. Instructions and regents are provided with the kit., depending on sample this will require prior salt/acid/acid-pepsin extraction.

**non-mammalian collagens may result in a reduced limit of detection. We recommend use of an assay standard matched to the species under assay.‍

Many customers have found that the straightforward sample processing and analysis of Sircol make it a good alternative to conventional hydroxyproline analysis.

Precautions : 

This kit is designed for research use only. Not for use in diagnostic procedures.
Kit requires access to a centrifuge, water bath / heated block, as well as a spectrophotometer/colorimeter capable of absorbance detection at 556nm.
Specific sample preparation protocols may require customer to provide further reagents, consult assay manual for further information.

 

Sircol Insoluble Collagen kit contents:‍

1. Sircol Dye Reagent (1x110ml)

2. Denatured Collagen Reference Standard (1x5ml, 1.0mg/ml)

3. Acid-Salt Wash Reagent (1x20ml)

4. Fragmentation Reagent (1x110ml)

5. Alkali Reagent (1x110ml)

6. 2ml screw-cap tubes for preparation of samples.

7. Assay kit manual

NB: Additional reagents may be required for sample preparation prior to assay. Consult manual or contact us for further details.

 

Product Codes

S2000  (100 measurements)

‍S6000  (Large Economy Kit, 400 measurements)