HOME
R&D
Cell Culture

Biocolor

Fastin™ - Elastin assay kit

Catalog No : F2000, F4000

Fastin is an easy-to-use, dye-based assay for measuring elastin from in-vivo and in-vitro sources. It can be used to quantify various elastin forms, spanning from immature tropoelastin to mature, 'insoluble' elastin fibers.
Colorimetric Detection (513nm) (Endpoint)
제품문의

Specifications

Assay range : 50 - 500µg/ml

Limit of Detection : 50µg/ml

Detection Method : Colorimetric Detection (513nm) (Endpoint)

Measurements per kit : 110 in total (allows a maximum of 48 samples to be run in duplicate alongside a standard curve).

Suitable Samples : 

In-vivo: tissues and fluids. Insoluble elastin will first require conversion to water soluble α-elastin using the oxalic acid reagent and extraction protocol supplied with the kit.  

In-vitro: Elastin produced by cells during 2D/3D cell culture.

Precautions : 

This kit is designed for research use only. Not for use in diagnostic procedures.
Kit requires access to a centrifuge, heated water bath or block, as well as a spectrophotometer or colorimeter capable of absorbance detection at 513nm.
Specific sample preparation protocols may require customer to provide further reagents, consult assay manual for further information.

 

Fastin elastin kit contents:‍

1. Dye Reagent (1x110ml)

2. α-elastin Reference Standard (1x5ml, 1.0 mg/ml soluble Bovine elastin)

3. Oxalic Acid (1x20ml) Precipitating Reagent (1x30ml)

4. Dye Dissociation Reagent (1x30ml)

5. Precipitating Reagent (1x30ml)

6. 1.5ml micro-centrifuge tubes for dye-labelling reaction.

7. Assay kit manual

NB: Additional reagents may be required for sample preparation prior to assay. Consult manual or contact us for further details.

 

Product Codes

F2000  (110 measurements)

F4000  (MultiPack Kit, 440 measurements)