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Blyscan™ - sulfated Glycosaminoglycan (sGAG) assay kit

Catalog No : B1000, B3000

Blyscan is an easy-to-use, dye-based assay for quantitative measurement of sulfated glycosaminoglycans (sGAG) & proteoglycans. Suitable samples include cells, tissues and fluids from a wide range of in-vivo and in-vitro sources.

Colorimetric Detection (656nm) (Endpoint)
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Specifications

Assay range : 2.5 - 50µg/ml

Limit of Detection : 2.5µg/ml

Detection Method : Colorimetric Detection (656nm) (Endpoint)

Measurements per kit : 110 in total (allows a maximum of 48 samples to be run in duplicate alongside a standard curve).

Suitable Samples : 

In-vivo: Solid samples, including cartilage, bone, connective tissue, tumour tissue

In-vivo: Liquid samples, including fluids such as urine, amniotic or synovial fluid.  

In-vitro: Solid samples, such as deposited ECM on 2D/3D culture surfaces.by enzymatic treatment

In-vivo: Liquid samples, Culture media during 2D/3D cell culture.

‍The assay requires that sulfated polysaccahrides or sGAGs are in a soluble form. A preliminary enzymatic extraction step is required for solid samples (enzyme not supplied with kit).

The assay is not suitable for use with samples containing alginates or that comprise degraded sulfated disaccharide fragments.

‍Precautions : 

This kit is designed for research use only. Not for use in diagnostic procedures.
Kit requires access to a centrifuge, as well as a spectrophotometer/colorimeter capable of absorbance detection at 656nm.
Specific sample preparation protocols may require customer to provide further reagents, consult assay manual for further information.

 

Product Codes

B1000  (110 measurements)

B3000  (Large Economy Kit, 440 measurements)