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P4 Primary Cell 4D-Nucleofector® X Kit S

Catalog No : V4XP-4032

For efficient transfection of specific primary cells, e.g. mouse B-cells or mouse immature DCs, in the 4D-Nucleofector® X Unit (20 µL format).
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Product Overview

 

The P4 Primary Cell 4D-Nucleofector® X Kit S is one of our five 4D-Nucleofector® Kits suited for transfecting primary cells in the 20 µL 16-well Nucleocuvette® Strip format when working with the 4D-Nucleofector® X Unit.

This kit has been determined to be optimal for transfecting following cell types:

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

 

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Benefits

 

  • High transfection efficiencies for primary cells
  • Cells maintain functionality post transfection
  • Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System

 

Applications

 

  • Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells
  • Medium throughput applications
  • Optimization of transfection conditions e.g. substrate titrations

 

Storage and Content

1 x 0.675 mL P4 Primary Cell Nucleofector® Solution
1 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well Nucleocuvette® Strips (20 μl)