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Cell Line Optimization 384-well Nucleofector® Kit

Catalog No : V5SC-9001

For high-throughput optimization of transfection conditions for cell lines lacking an Optimized Protocol for the 384-well HT Nucleofector® System
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Product Overview

 

The Cell Line Optimization 384-well Nucleofector® Kit is suited for high-throughput optimization of transfection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT Nucleofector® System.

Different conditions can easily be tested within one experiment using a 384-well Nucleocuvette® Plate and our pmaxGFPTM Vector as positive control. Our three Cell Line Nucleofector® Solutions SE, SF and SG are tested in combination with a pre-selected set of programs plus controls. A subsequent optional fine tuning of the Nucleofection conditions can be performed with the help of our Scientific Support Team.

Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.  

 

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Benefits

 

  • High transfection efficiencies for cell lines
  • Automated high-throughput electroporation
  • Convenient and rapid determination of optimal Nucleofection conditions for a broad range of cell lines within one experiment
  • Optimal Nucleofection conditions determined on the 384-well HT Nucleofector® System are transferable to the 4D-Nucleofector® System or the 96-well Shuttle® Add-On

 

Applications

 

  • Transfection of low cell numbers in the range of 2 x 104 to 1 x 106 cells
  • Determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 384-well HT Nucleofector® System

 

Storage and Content

2.25 mL Nucleofector® Solution SE

2.25 mL Nucleofector® Solution SF

2.25 mL Nucleofector® Solution SG

3 x 0.15 mL Supplement 1

150 µg pmaxGFPTM Vector (1 μg/μL in 10 mM Tris pH 8.0)

1 x 384-well Nucleocuvette® Plate