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Cell Line Nucleofector® Kit L

Catalog No : VCA-1005

For efficient transfection of cell lines, e.g. 3T3-L1 ad, DU145, EL4, IMR-32 or MDCK, in the Nucleofector® I/II/2b Device
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Product Overview

 

The Cell Line Nucleofector® Kit L is one of our five Nucleofector® Kits suited for transfecting cell lines in the Nucleofector® 2b System.

This kit has been determined to be optimal for transfecting following cell types:
3T3-L1 ad
A-10
BHK-21
BxPC-3
DU145
EL4
FDC-P1
GH3 H9c2 (2-1)
HeLa S3
IMR-32
KG-1a
L-428 (DSMZ)
MDCK
MDCK II (ECACC)
MOLT-4
MV-4-11
NTERA-2 cl.D1
WEHI-231

The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.

 

 

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Benefits

 

  • High transfection efficiencies in difficult-to-transfect cell lines

 

Applications

 

  • Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells 
  • Low-throughput transfection in single cuvette format

 

Storage and Content

2.25 mL Nucleofector® Solution L
0.5 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL),
25 Single use pipettes