Product Overview

In addition to various cell type-specific kits for primary neurons, we also provide a cell group-specific Basic Nucleofector^® Kit. This kit is suited to determine the optimal transfection conditions for any mammalian neuron type that lacks a cell type specific Optimized Protocol for the Nucleofector^® 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector^® Programs is tested in combination with the Basic Nucleofector^® Solution for neurons. 

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

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Benefits

• Optimization can be performed within 1 experiment 
• Detailed protocols guiding through the optimization procedure 
• Optional result fine tuning with help of our Scientific Support Team 
• Transfection efficiencies up to 92%
• Viabilities up to 80%

Applications

• Kit suited for various neuron types from different mammalian species

Storage and Content

2.25 mL Nucleofector^® Solution for Primary Mammalian Neurons
0.5 mL Supplement 1
30 µg pmaxGFP^TM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL)
25 Single use pipettes