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Human Hepatocytes, Cryopreserved, Plateable and Interaction Qualified

Catalog No : HUCPI

Cryopreserved human hepatocytes, plateable and interaction qualified are characterized for 3 major mechanisms of drug-drug interactions: transporter activity, enzyme activity, and induction potential (guaranteed to exhibit a 4 fold or greater change in induction activity), providing you with one product to meet more of your hepatocyte DDI study needs. Each ampule contains >5 million cells.
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Product Overview
Clinically relevant drug-drug interactions (DDI) are a serious concern for any new drug development project.

To better support capturing the multiple mechanisms of DDI potential using cryopreserved primary human hepatocytes, we offer Interaction Qualified Cryopreserved Human Hepatocytes (Catalog HUCPI) which are characterized for 3 major mechanisms of DDI:

- Transporter activity
- Enzyme activity
- Enzyme gene induction potential. 
Interaction qualified cryopreserved human hepatocytes have the following features and benefits:

 

Benefits

- Use the same donor for basal clearance, transport, and induction studies
- Actual rates of transport reported rather than relative rates for less ambiguity
- Large lots mean fewer rounds of testing so you can focus on results
- Prequalified in Lonza media for better reproducibility

 

Features

- Confluent monolayer with cobblestone morphology, distinct nuclei, and tight junctions for 5 days or more
- Inducibility of enzyme activity for CYP3A4, CYP2B6, and CYP1A2 (guaranteed 4-fold or greater activity change)
- Inducibility of mRNA for CYP3A4, CYP2B6, CYP1A2, and CYP2C8 genes
- Actual rate of uptake or efflux for OATP1B1/3, OCT1/2, NTCP, and BSEP** transporters
- Differences in passive vs. active uptake for OATP1B1/3, OCT1/2, and NTCP transporters.
- Basal metabolism for 8 CYPs, SULT, UGT, and aldehyde oxidase
- Plated low-turnover clearance for CYP2C9, CYP2D6, and CYP3A4
- Complementary thawing, plating, and maintenance medium available
- > 5 million viable cells/vial
- Characterized for long-term culture potential (> 7 days)
- Characterized for spheroid formation potential



Benefits
Use the same donor for basal clearance, transport, and induction studies
Actual rates of transport reported rather than relative rates for less ambiguity
Large lots mean fewer rounds of testing so you can focus on results
Prequalified in Lonza media for better reproducibility
Applications
DMPK studies
Drug-Drug interaction studies
Low clearance metabolism studies
Drug uptake and efflux
Disease modeling
Xenotransplantation
Short term cellular toxicity studies
3D cell culture


Storage and Content
Cryopreserved ampule of HUCPI containing >5 million viable cells