Product Overview

ViaLight^® Plus BioAssay Kits are designed to provide unprecedented speed and sensitivity for cytotoxicity and cell proliferation studies. The ViaLight^® Assay incorporates bioluminescent detection of cellular ATP as a measure of viability. Measurement of ATP is the most accurate, effective, and direct way of determining the number of living cells in culture.

The ViaLight^® Plus BioAssay is designed to deliver a high, stable luminescent signals for an extended period of time for greater experimental design flexibility. The easy two-step, non-radioactive assay is scalable for high-throughput applications in both 96-well and 384-well formats. The assay can be run on a variety of luminometers.

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Benefits

• Fast - Results from a 96 well plate can be processed and analyzed in as little as 10 minutes.

• Sensitive - Detect as few as 10 cells allowing for lower seeding densities and more assays.

• Convenient - Simply add two reagents directly to your culture well and read. No cell washing or medium removal required.

• Flexible - Dynamic range of 5 decades and excellent performance with both adherent or suspension cultures allows you to design the experiment for your needs.

• Robust - Stable bioluminescent signal (half-life >5 hours) facilitates manual or batch processing. And, unlike MTT or Alamar Blue assays, ViaLight^® Bioassays are not prone to interference due to culture condition changes.

Storage and Content

10,000 tests (Sufficient for 100 plates)

• 10 x LT27-213 ATP monitoring reagent plus, Lyophilized
• 10 x LT27-080 Assay buffer, 100 mL bottles
• 5 x LT27-077 Cell lysis reagent, 100 mL bottles