Product Overview

The X-VIVO^® 10 Media formulations are designed to support the generation of LAK cells in a serum-free environment. The original protocols involved the incubation of patient or normal donor peripheral blood lymphocytes (PBL) at 1.0 – 3.0 × 10⁶ cells/ml for a period of 3 days in the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK cell generation is achieved when peripheral blood lymphocytes are incubated for 3 – 10 days at a density of 1.0 – 6.0 × 10⁶ cells/ml in the presence of 100 – 1,000 Cetus units of rIL-2.

X-VIVO^® 10, catalog number 04-380Q contains L-glutamine, gentamicin, and phenol red. 

THIS PRODUCT IS NOT FOR USE IN GMP MANUFACTURING, NOR HUMAN OR ANIMAL IN VIVO USE, INCLUDING USE AS A DILUENT OR AS AN EXCIPIENT, OR FOR DIAGNOSTIC USE. This product is for research use only.

If you require GMP grade media, contact Lonza for more details.

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Benefits

• Superior media for most hematopoietic cells types including dendritic cells, lymphocytes, monocytes, macrophages and granulocytes.
• Formulations only contain human derived components
• Highly cited in scientific literature
• Clinical proven formulations providing process safety and constancy
• High productivity resulting in a high viable cell yield
• Serum Free media result in a more defined process and result in limited immune response
• Efficient transgene expression
• Desirable T-cell phenotype

Applications

• Proliferation of peripheral blood lymphocytes
• Proliferation of tumor infiltrating lymphocytes
• Cryopreservation of human tissue
• Cultivation of human monocytes and macrophages
• Cultivation of stem cells
• Cultivation of dendritic cells

Storage and Content

1L bottle