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HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM® Media

Catalog No : CC-2519

Cryopreserved ampoule of Human Umbilical Vein Endothelial Cells (HUVEC) from pooled donors, containing ≥ 500,000 cells
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Product Overview

 

Lonza's cryopreserved HUVECs are shipped as frozen primaries. They are guaranteed to express CD31/105 and survive in culture through 15* population doublings (*except HUVEC-XL guaranteed through 5 PD).

All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Our pooled vs. single donor options allow researchers to study or exclude donor variability from their experiments. Depending upon the study, pooled versions are typically utilized to reduce donor variability while single donor options allow researchers to assess donor-specific differences.

HUVEC-XL size consists of 10 million cells/amp achieved by pooling donors. This option is designed to support studies that require large volume of cells while still offering cost-benefits to researchers.

Not All HUVECs Are Created Equal – Which Type Is Suitable for Your Research?

  • CC-2517 (single donor), CC-2519 (pooled donor) - Both isolated in EGM® Media, in absence of defined growth factors. Contains BBE. No exogenous VEGF.
  • CC-2935 (single donor) - Isolated in EGM®-Plus Media: Improved version of part number CC-2517. Offers faster proliferation rates than HUVEC isolated in EGM® Media. No exogenous VEGF. 
  • C2517A (single donor), C2519A (pooled donor), 00191027 (XL size) - Isolated in EGM®-2 Media, in presence of growth factors such as VEGF, rhFGF, rhEGF, and others. Does not contain BBE.
  • C2517AS (single donor), C2519AS (pooled donor) - Pre-screened HUVECs in EGM®-2 Media. Screened for angiogenesis markers and test positive for expression of eNOS, Axl, Tie-2 and VEGFr2.

 

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Benefits

 

  • Guaranteed through 15 population doublings 
  • Single or pooled donors available 
  • All cells test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis provided for every cell lot

 

Applications

 

  • Wound healing
  • Angiogenesis
  • Tissue engineering
  • Inflammation
  • Oncology
  • Pharmacology
  • Vascular modeling
  • Transfection 

 

Storage and Content

1 x cryopreserved ampoule HUVEC (pooled donor) containing ≥500,000 cells