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NHDC - Human Dendritic Cells, 2.5 Million

Catalog No : CC-2701

Cryopreserved ampoule of Normal Human Dendritic Cells (NHDC) containing ≥2.5 million cells
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Product Overview

 

Dendritic Cells (DCs) act as the messenger cells of the immune system by processing antigens from pathogens and presenting the antigens to T cells to initiate an immune response. PoieticsTM Normal Human Dendritic Cells are tested for a panel of specific surface markers, including CD11c+, CD86+, CD80+. HLADR+, and CD14- to provide detailed characterization of the cells. These cells can survive up to 7 days in culture with the proper cytokines (IL-4 and GM-CSF) to maintain the cells as immature dendritic cells (iDCs). Cells can be further differentiated to mature dendritic cells with the addition of TNF-α and IL-1β. 

Applications: Antigen presentation and uptake Primary Research Pharmacology Drug-induced immunosuppression of cytokine secretion

Cryopreserved Poietics® Normal Human Dendritic Cells (NHDC) are guaranteed to contain ≥3 million viable cells per ampoule. Expression levels are tested for CD80, CD86, HLA-DR, CD11c, and CD14 and provided for information only. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Recommended Medium: LGM-3TM Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4.

Lonza guarantees the performance of Clonetics®/Poietics® Cells only if appropriate Clonetics®/Poietics® Media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

 

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Benefits

 

  • All cells test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis provided for every cell lot

 

Storage and Content

1 x cryopreserved ampoule of NHDC containing ≥2.5 million cells