Product Overview

Human Cardiac-Related Fibroblasts include Aortic Adventitial Fibroblasts (AoAF) isolated from the tunica external of the ascending or descending aorta and cardiac fibroblasts (NHCF-A and -V) isolated from the atrium or ventricle.  Cells are isolated from normal, adult heart tissue. Cryopreserved cells are shipped at passage 2.

Clonetics^TM NHCF-V & -A are guaranteed through 5 population doublings and express ≥90% collagen I and ≤10% Von Willibrand factor VIII after thaw. Clonetics^TM AoAF are guaranteed through 10 population doublings and are negative for alpha smooth muscle actin. All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.  

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Applications

• Vascular diseases
• Neointimal formation
• Vascular remodeling
• Atherosclerosis
• Hypertension
• Myocardial repair
• Extracellular maintenance during cardiac cell / tissue growth
• Cardiac Fibrosis Paracrine signalling
• Toxicology
• Gene expression

Storage and Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial