Product Overview

Irradiated Mouse primary embryonic fibroblasts (iMEF) are dissociated from day 14 and 15 CD1 mouse embryos, expanded and then inactivated using gamma radiation.

Each vial contains at least 2 million cells. iMEF are convenient and easy to use “feeder cells” commonly co-cultured with embryonic and pluripotent stem cell cultures.

Recommended Media: Lonza's DMEM High Glucose w/ L-Gln, 500 mL (Part 12-604F) containing 10% FBS (Sigma-Aldrich Part F6178)

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Benefits

• Ready-To-Use 
• No tissue dissection or animal handling required 
• Increased data reproducibility
• Characteristic morphology and staining tests confirm tissue origin and cell type
• Negative for mycoplasma, bacteria, yeast and fungi
• Certificate of Analysis (CoA) is available for every batch 

Applications

• Serve as “feeder cell” monolayer in embryonic (ES) and induced pluripotent (iPSC) stem cell cultures 
• Secrete important growth factors into culture medium to support ES and iPSC undifferentiated state
• Act as cellular matrix to support ES cell growth 

Storage and Content

≥ 2 million cryopreserved, gamma irradiated cells per ampule