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P2 Primary Cell 96-well Nucleofector® Kit

Catalog No : V4SP-2096

For medium-throughput transfection of primary cells, e.g. mouse macrophages or NHDF, when working with the 4D-Nucleofector® 96-well Unit.
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Product Overview

 

The P2 Primary Cell 96-well Nucleofector® Kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 4D-Nucleofector® 96-well Unit.

This kit has been determined to be optimal for transfecting following cell types:

  • Macrophage (mouse)
  • NHDF (human), neonatal

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 1 x 96 or 10 x 96 reactions.

 

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Benefits

 

  • High transfection efficiencies for primary cells
  • Compatible with liquid handling systems for automated high-throughput electroporation
  • Conditions are transferable between 4D-NucleofectorTM System, 4D-Nucleofector® 96-well Unit and 384-well Nucleofector® System

 

Applications

 

  • Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells
  • High-throughput applications,e.g. s creens of arrayed cDNA, RNAi or CRISPR libraries

 

Storage and Content

2.25 mL P2 Primary Cell Nucleofector® Solution
0.5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
1 x 96-well Nucleocuvette® Plate (20 μl)