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SF Cell Line 4D-Nucleofector® X Kit L

Catalog No : V4XC-2012

For efficient transfection of difficult-to-transfect cell lines, e.g. K-562, Neuro-2A or RAW264.7, in the 4D-Nucleofector® X Unit (100 µL format).
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Product Overview

 

The SF Cell Line 4D-Nucleofector® X Kit L is one of our three 4D-Nucleofector® Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette® Vessel format when working with the 4D-Nucleofector® X Unit.

This kit has been determined to be optimal for transfecting following cell types:

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 12 or 24 reactions.

 

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Benefits

 

  • High transfection efficiencies in difficult-to-transfect cell lines

 

Applications

 

  • Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells
  • Low throughput Applications s

 

Storage and Content

2 x 0.675 mL SF Cell Line Nucleofector® Solution
2 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
12 x 100 µL Nucleocuvette® Vessel
12 x Single use pipettes