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P4 Primary Cell 384-well Nucleofector® Kit

Catalog No : V5SP-4002

For high-throughput transfection of primary cells, e.g. moues B-cells or immature DCs, when working with the 384-well HT Nucleofector® System.
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Product Overview

 

The P4 Primary Cell 384-well Nucleofector® Kit is one of our five kits suited for high-throughput transfection of primary cells when working with the 384-well HT Nucleofector® System.

This kit has been determined to be optimal for transfecting following cell types:

  • B cell (mouse)
  • DC (mouse), immat.
  • MEF (mouse)

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 2 x 384 or 10 x 384 reactions.

 

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Benefits

 

  • High transfection efficiencies for primary cells
  • Cells maintain functionality post transfection 
  • Seamless integration into automated liquid handling environments
  • Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System

 

Applications

 

  • Transfection of low cell numbers down to 2 x 104 primary cells
  • High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries

 

Storage and Content

22.5 mL P4 Primary Cell Nucleofector® Solution,
5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μlL in 10 mM Tris pH 8.0)
2 x 384-well Nucleocuvette® Plate (20 μL)