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Primary Cell Optimization 384-well Nucleofector® Kit

Catalog No : V5SP-9001

For high-throughput optimization of transfection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT Nucleofector® System
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Product Overview

 

The Primary Cell Optimization 384-well Nucleofector® Kit is suited for high-throughput optimization of transfection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT Nucleofector® System.

Complete Optimization: Different conditions can easily be tested within one experiment using a 384-well Nucleocuvette® Plate and our pmaxGFPTM Vector as positive control. Our five Primary Cell Nucleofector® Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. A subsequent optional fine tuning of the Nucleofection conditions can be performed with the help of our scientific support team.

Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector® Solutions and Nucleofector® Programs. Basic Protocols are available for following cell groups:

  • Endothelial cells (mammalian)
  • Epithelial cells (mammalian)
  • Fibroblasts (mammalian)
  • Glial cells (mammalian)
  • Neurons (mammalian)
  • Smooth muscle cells (mammalian)
  • Stem cells (human)

Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.

 

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Benefits

 

  • High transfection efficiencies for primary cells
  • Automated high-throughput electroporation
  • Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
  • Optimal Nucleofection conditions determined on the 384-well HT Nucleofector® System are transferable to the 4D-Nucleofector® System or the 96-well Shuttle® Add-On

 

Applications

 

  • Transfection of low cell numbers in the range of 2 x 104 to 1 x 106 cells
  • Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT NucleofectorTM System

 

Storage and Content

2.25 mL Nucleofector® Solution P1

2.25 mL Nucleofector® Solution P2

2.25 mL Nucleofector® Solution P3

2.25 mL Nucleofector® Solution P4

2.25 mL Nucleofector® Solution P5

4 x 0.15 mL Supplement 1

1 x 0.15 mL Supplement 3

150 µg pmaxGFPTM Vector (1 μg/μL in 10 mM Tris pH 8.0)

1 x 384-well Nucleocuvette® Plate