Product Overview

The Nucleofector^® 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. Due to significant diversity between different human stem cell clones, we have developed two Nucleofector^® Kits (1 and 2) and an easy-to-use Nucleofector^® Starter Kit. The Human Stem Cell Nucleofector^® Starter Kit is used to determine which one of our two Nucleofector^® Kits is best suited for transfecting a specific primary human stem cell clone on Nucleofector^® 2b System. It is suitable for 18 optimization reactions.

The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.

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Benefits

• High transfection efficiencies for primary cells
• Cells maintain functionality post transfection

Applications

• Transfection of cell numbers in the range of 2 × 10⁵ to 2 × 10⁷ cells 
• Low-throughput transfection in single cuvette format

Storage and Content

0.9 mL Human Stem Cell Nucleofector^® Solution 1
0.9 mL Human Stem Cell Nucleofector^® Solution 2
2 x 0.2 mL Supplement 1
2 x 30 µg pmaxGFP^TM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
20 Aluminum cuvette (100 µL)
20 Single use pipettes