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Basic Nucleofector® Kit for Primary Mammalian Smooth Muscle Cells

Catalog No : VPI-1004

For transfection of mammalian smooth muscel cells lacking a cell type specific Optimized Protocol for the Nucleofector® I/II/2bDevice
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Product Overview

 

In addition to various cell type-specific kits for primary smooth muscle cells, we also provide a cell group-specific Basic Nucleofector® Kit. This kit is suited to determine the optimal transfection conditions for any mammalian smooth muscle cell type that lacks a cell type specific Optimized Protocol for the Nucleofector® 2b Device (or older generations I or II). In a first experiment a selection of different NucleofectorTM Programs is tested in combination with the Basic Nucleofector® Solution for smooth muscle cells. 

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

 

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Benefits

 

  • Optimization can be performed within 1 experiment 
  • Detailed protocols guiding through the optimization procedure 
  • Optional result fine tuning with help of our Scientific Support Team
  • Transfection efficiencies up to 95%
  • Viabilities up to 96%

 

Applications

 

  • Kit suited for smooth muscle cells from different mammalian species and various organs 
  • Already tested for porcine vascular smooth muscle cells and coronary artery smmoth muscle cells (CASMC)

 

Storage and Content

2.25 mL Nucleofector® Solution forPrimary Mammaliean Smooth Muscle Cells
0.5 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL)
25 Single use pipettes