Adherent Nucleofection^® Using the Y Unit

Electroporation-based methods have so far required cells to be in suspension for transfection. The Nucleofector^® Technology entered a new era and allows direct Nucleofection^® Reactions of cells in adherence. Cells which typically grow in adherence in cell culture, can be kept and transfected by Nucleofector^® Technology in their physiological state. The Y Unit of the 4D-Nucleofector^® System works with disposable conductive polymer Dipping Electrode Arrays that can be inserted into standard 24-well culture plates for Nucleofection^® Procedure.

The 4D-Nucleofector^® Y Unit is one of the four functional modules of the 4D-Nucleofector^® System.

There are cell type-specific Optimized Protocols or recommendations available in our knowledge database. In these Optimized Protocols the best Nucleofection^® Conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

✔️ Technical Specifications

  Dimensions: (w x h x d): 24.5×10.5×28 cm / 9.7×4.1×11.0

  Weight: 5.0 kg (11.0 lb)

  Power: 100 - 240 VAC

  Frequency 50 - 60 Hz

  Consumption: 0 VA (power off) / 20 VA (idle) / 140 VA (busy - during transfection)

  Fuse: T 2.5A, L250V

  Operating temperature: +15°C up to +40°C (59°F - 104°F)

  Environment non condensing

  Voltage limitation: 1500 V

  Current limitation: 100 A

✔️ Compatibility

This functional module can be operated with all versions of the Core Unit (part code AAF-1001B, AAF-1002B, AAF-1003B)

✔️ Benefits

Pre- and post Nucleofection^® culture in 24-well culture plates

• Nucleofection^® of cells at any time point during this culture period, i.e. at a later developmental stage
• Transfection efficiencies up to 70% combined with high viabilities
• Compatible with Clonetics^® Primary Animal Neurons