Specifications

Assay range : 10 - 150µg/ml or 10 - 500µg/ml (depending on setup)

Limit of Detection : 10µg/ml

Detection Method : Colorimetric Detection (556nm) (Endpoint)

Measurements per kit : 110 in total (allows a maximum of 48 samples to be run in duplicate alongside a standard curve).

Suitable Samples : 

Soluble* collagens from mammalian**:

In-vivo: tissues, cartilages and fluids.

‍In-vitro: 2D/3D culture extracellular matrices. Customers wishing to analyse conditioned media should first consider the new Sircol 2.0 assay kit.

*Collagens must be in soluble form, depending on sample this will require prior salt/acid/acid-pepsin extraction.

**non-mammalian collagens may result in a reduced limit of detection. We recommend use of a collagen standard matched to the species under assay.

Many customers have found that the straightforward sample processing and analysis of Sircol make it a good alternative to conventional hydroxyproline analysis.

Precautions : 

This kit is designed for research use only. Not for use in diagnostic procedures.

Kit requires access to a centrifuge, as well as a spectrophotometer/colorimeter capable of absorbance detection at 556nm.
Specific sample preparation protocols may require customers to provide further reagents, consult assay manual for further information.

Sircol kit contents

1. Sircol Dye Reagent (1x110ml)

2. Collagen Reference Standard (1x5ml, 0.5mg/ml soluble Bovine collagen)

3. Acid-Salt Wash Reagent (1x20ml)

4. Collagen Isolation and concentration Reagent (1x20ml)

5. Alkali Reagent (1x110ml)

6. Acid-Neutralising Reagent (1x20ml)

7. 1.5ml micro-centrifuge tubes for dye-labelling reaction.

8. Assay kit manual

NB: Additional reagents may be required for sample preparation prior to assay. Consult manual or contact us for further details.

Product Codes

‍S1000  (110 measurements)

S5000  (Large Economy Kit, 440 measurements)