Product Overview

Dendritic Cells (DCs) act as the messenger cells of the immune system by processing antigens from pathogens and presenting the antigens to T cells to initiate an immune response. Poietics^TM Normal Human Dendritic Cells are tested for a panel of specific surface markers, including CD11c+, CD86+, CD80+. HLADR+, and CD14- to provide detailed characterization of the cells. These cells can survive up to 7 days in culture with the proper cytokines (IL-4 and GM-CSF) to maintain the cells as immature dendritic cells (iDCs). Cells can be further differentiated to mature dendritic cells with the addition of TNF-α and IL-1β. 

Applications: Antigen presentation and uptake Primary Research Pharmacology Drug-induced immunosuppression of cytokine secretion

Cryopreserved Poietics^® Normal Human Dendritic Cells (NHDC) are guaranteed to contain ≥3 million viable cells per ampoule. Expression levels are tested for CD80, CD86, HLA-DR, CD11c, and CD14 and provided for information only. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Recommended Medium: LGM-3^TM Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4.

Benefits

• All cells test negative for mycoplasma, bacteria, yeast, and fungi
• HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots
• Certificate of Analysis provided for every cell lot

Storage and Content

1 x cryopreserved ampoule of NHDC containing ≥2.5 million cells